The innovation of this research is to combine the traditional immunohistochemical staining technique, which can accurately label multiple macromolecules in cells, with multicolor three-dimensional random optical reconstruction super-resolution microscopy (3D-dSTORM), which makes it possible to image cell wall polysaccharides with high sensitivity and resolution. dSTORM super-resolution technology, invented by Chinese scientist Xiaowei Zhuang, advances microscopy resolution to a few nanometers, which is nearly two orders of magnitude higher than the limit of optical diffraction. Nearly two orders of magnitude higher than the optical diffraction limit, it has greatly facilitated biomedical research